What Do Pizza Boxes, Raincoats and Frying Pans Have in Common?
Detecting PFAS in everyday products and our environment
PFAS, per and polyfluoroalkyl substances, are a class of synthetic chemicals that you may not have heard of, but have certainly been in contact with. These chemicals have unique and useful characteristics and can be used to make products heat and stain resistant, non-stick and water repellent. They are present in a myriad of consumer products, from the pans we use to cook our food, to our raincoats and even some dental floss. Unfortunately, they are very stable compounds that accumulate and persist in our environment and have been linked to a number of health risks. Eurofins companies have been at the forefront of developing methods to test for these compounds.
PFAS comprise a group of thousands of synthetic chemicals that have been in commercial production since the 1940s. They can be found in everyday products like non-stick cookware, fire retardants, stain and water repellents, some furniture, waterproof clothes, pizza boxes, food packaging, carpets and textiles, firefighting foam, rubbers and plastics, electronics and even some dental floss. The primary sources of PFAS in the environment are manufacturing facilities and in Sweden particularly, firefighting foams used at firefighting training sites. PFAS are largely unsusceptible to degradation and remain in the environment almost indefinitely. Their water solubility allows them to penetrate soil.
Some PFAS accumulate over time to increased concentrations in humans, animals and the environment. They are dispersed through air and water, to such an extent that they have even been found in the environment of the Arctic and its polar bears, and open ocean waters. PFAS have also been found in fish, shellfish, vegetables and other produce grown in contaminated soil or water. The Centers for Disease Control and Prevention (CDC) found PFOA, a specific PFAS, in the blood of 98% of Americans, as well as in breast milk and umbilical cord blood.
The health effects of the various kinds of PFAS are under constant research, however evidence has linked exposure to some of the chemicals to developmental issues, cancer, liver damage, immune system disruption, resistance to vaccines, thyroid diseases, impaired fertility and high cholesterol. PFAS have been dubbed “possibly carcinogenic” to humans by the US Environmental Protection Agency (EPA) and the International Agency for Cancer Research (IARC).
With public concern regarding the health effects of PFAS outpacing the regulatory framework to address these complex chemicals, the scientific community stepped in and worked to develop an approach to assess the prevalence of PFAS in the environment. Eurofins Environment USA became the first commercial laboratory to test environmental samples for PFAS in the USA. In 2016, Eurofins Environment Testing Sweden AB became the first accredited commercial laboratory to be able to detect PFAS in water at LOQs (limit of quantification) of 0.3 parts per trillion (ppt). One part per trillion is equivalent to a single grain of sand in an Olympic sized swimming pool. These levels are even lower than those required by the European Union Water Framework Directive (WFD). PFAS testing is a field where change and learnings are developing rapidly and being at the frontier of research is extremely important. Eurofins Sweden remains at the forefront of research in the area and is involved in a long-term research project, EnForce, with Örebro University in Sweden.
Of the several thousand of PFAS chemicals, laboratories are only able to analyse for a couple of dozen of known PFAS compounds, as many remain largely unidentified or unknown currently. Eurofins’ teams of expert scientists and its investment in state-of-the-art equipment has made identifying some of these unknown PFAS in the environment and their sources possible. In 2016, Eurofins introduced the Total Oxidizable Precursor Assay technique, which allows for the measurement of unknown PFAS by identifying precursors of PFAS that have the potential to transform, when oxidation occurs in the environment, into the PFAS compounds that can be analysed. Eurofins Environment USA have been called upon to support the validation of several US EPA methods to measure PFAS in potable and non-potable water.
In addition to water testing, Eurofins companies have more recently expanded their PFAS testing across other matrices, including food, and feed. Additionally, through a Eurofins empowerDX and Eurofins Environment Testing collaboration, the companies launched the first direct-to-consumer PFAS Exposure™ Test to identify the chemicals in blood samples.
Today, a growing concern regarding PFAS is how they are spread through air pathways. PFAS are emitted into the air from manufacturing facilities or facilities that treat waste of products that comprise PFAS. Eurofins companies have pioneered sampling and analytical techniques to collect and analyse PFAS from these sources, including soil vapour and stack emissions, and monitor ambient outdoor and indoor air for these chemicals, to collect the information we need to keep the air around us safer. Such specific analysis requires unique and highly specialised equipment, often only found in research laboratories and academia. Eurofins companies maintain more of this specialised equipment than any commercial laboratory in the USA and are using it to develop techniques with the aim of closing some of the data gaps that currently exist in relation to PFAS.
The science behind
The Total Oxidizable Precursor Assay technique is a chemical oxidation of PFAS precursors yielding measurable perfluorinated carboxylic acids. The aim is to assess the “unknown pool” of compounds that, with time, may form other, more stable PFAS in the environment.
Eurofins Environment Testing laboratories developed LC-MS/MS analysis (Liquid Chromatography with tandem mass spectrometry) for several classes of PFAS and have expanded both the list of PFAS and matrices analysed. These matrices include groundwater, soil and sediment, tissues, serum and foodstuffs, but have recently expanded to textiles and even the air one breathes. LC-MS/MS is a powerful analytical technique that combines the separating power of liquid chromatography with the highly sensitive and selective mass analysis capability of triple quadrupole mass spectrometry. With these tools, Eurofins achieves industry leading detection limits: as low as parts-per-trillion (ppt) detection limits for aqueous matrices and less than a part-per-billion (ppb) for solids and tissues.